Osmosis in Different Concentrations

I am going to analyze osmosis when murphy is popd in incompatible duckings of saccharose. I am aiming to witness osmosis in 5 different stringencys of saccharose. I entirelyow for aim 5 change tightfistednesss so that I realize a wider splay to comp argon the results, and check that I dont pull in whatsoever anomalies Prediction Osmosisis the process of distri providedion of peeing supply supply molecules from a weaker solution into a stronger solution, done a out(p)fitpermeable tissue layer.The tiny pores in the membrane of the murphyes go forthing allow the pissing molecules to go in and surface of the potato electric cellular telephone, depending on the tightfistedness gradient among the potato and the saccharose solution. If thewater system tightnessis bring low in the potato than in the sucrose solution, indeed water get out promontory from the sucrose solution into the potato, and it allow for consume system of loads.If in that loca tion is a higher tautness of water in the potato, thusly the water go out go out of the potato and into the sucrose solution, as osmosis is the political campaign of water molecules from a region of high concentration to a region of low concentration, through a semi permeable membrane. So, I predict that the higher the concentration of sucrose, the light the load of the potato as water from the potato milds into the sucrose solution. Predicted graphical record of results IMAGE Apparatus * 6 try on renders * 6 different concentrations of sucrose solutions * stump spud potato cutter/ ticker * Digital bill scales * Measuring cylinders * Knife * Test tube racks * wind paper Method I testament shoot 1 piece of potato in severally tube. As on that point are 15 tubes I will deem to calculate how numerous pieces I will need altogether. I conjecture this would be a suitable calculation 115=15. So, I will pierce out whatsoever pieces of a potato using a core, and thusly c ut 15 slices with a knife. Then I will stride 10ml of each of the 6 sucrose solutions using a standard cylinder. I will be given these different concentrated solutions in the general testing ground.I will label the tubes 1 to 15 and fill each one with 10ml of the solution. vacuum tube 1-3 will rich person a concentration of 0 Tube 4-6 will have a concentration of 0. 4 Tube 7-9 will have a concentration of 0. 8 Tube 10-12 will have a concentration of 1. 2 Tube 13-15 will have a concentration of 1. 4 I will footfall each slice on the measure scales to identify each potato slice with its metric pack unit onward and afterward immersion in the sucrose. Having thrifty each slice on the scale and save its weight, I will then place the slices in the test tube containing the solution for 24 hours.Then I will empty the solution from the test tube by pouring it in the sink and omit the potatoes by hand and place them one by one on the scale. For accuracy, I will addle sure that I wipe off any excess solution on the scale before placing each new slice on it. I will go in the weight after placing them in the solution for 24 hours. This process will be repeated for tubes 1 to 15 and the results will be noted in a table and then p striationted on a line graph. I will refer this a fair test by only varying the concentration of sucrose and the size of the potato slices, but keeping e truly(prenominal)thing else the aforestate(prenominal).The same potato must be used for the square sample or another(prenominal)wise, the results would differ as the age and sizes might be different, which means one potato might have more than water in it than the other. I will use a 10ml measuring cylinder so that I can faultlessly measure the exact amount of sucrose needed. As the cylinder measures different sucrose solutions, it has to be serve out each time I measure another 10ml of sucrose for the following(a) tube, because it whitethorn be contaminated with the di fferent sucroses.All the tubes will be kept for the same time, in the same place, so that the un dominationlable temperature would not affect the tubes separately. I will not be able to control * Temperature because I wont be in the laboratory for 24 hours, and the temperature could change in the night, or morning. *Temperature of solution- because I was not able to obtain a thermometer and I was not in the laboratory for 24 hours so it could change. * Weighing scales because these are digital and therefore, it produces results by itself. I will be able to control Concentrations of sucrose they are already measured * Time I will do the experiment, and come back the next day at the same time, and promptly record the results *weight of the potato- I weighed the potato slices before the experiment. For prophylactic in the laboratory, I will be genuinely careful using the sharp knife which I will be using to cut the potatoes with. I will make sure that I have an overall so that my c takehes dont get dirty if the solutions spills. I will suppose not to put potatoes or sucrose in my mouth as they might have been infected by chemicals in the lab, which are poisonous.I will measure the weight of the potato in grams, and the concentration of sucrose in percentage. I am expecting e verything to work out well as I have a perfectly good method but if I feel that the results may be wrong, I will repeat my experiment. Preliminary results These are my results This experiment supports my blueprint and prediction. The carrot gains weight in normal water, and decreases weight in concentrated sugar solutions. By doing this experiment, I believe that my method is good, and it will work. I think I should specifically be aware of the scales, because they falsify a lot.However, this was carried out at home, so the grooming scales may have not been so accurate, as accuracy is not very important in cooking. Results these are my first results. They proved to be wrong. This f ault would have been made my human. I assume that I used two different scales, and have got all the weights jumbled up. The weight taken at the source is also not correct, so I may have made a mistake skilful from the start. Concentration of sucrose. Weight before Weight after 0 1. 28 0 1. 33 0 1. 29 0. 4 1. 29 0. 4 1. 27 0. 4 1. 2 0. 8 1. 31 0. 8 1. 3 0. 8 1. 3 1. 2 1. 29 1. 2 1. 37 1. 2 1. 27 1. 6 1. 26 1. 6 1. 3 1. 6 1. 31 Conclusion I found out that as the concentration of sucrose join ons, the weight of the potato decreases. In water it gained 1. 26 grams, but in 1 Sucrose solution, it lost 0. 16 grams. My prediction supports my conclusion. I have calculated the average change in weight to have a simple, clear head where the experiment is leading me to. Instead of having a intumescent number of weights, I combined them into one by averaging them.I found the average by adding the weight gain/loss for each tube and divided it by three. To find the percentage, I multiplied that decimal by 100. I drew a line graph, and then a line of best fit, which is sloping downwards, negatively. This proves that the weight is getting lower as the percentage of the sucrose concentration is getting higher. In my probe I found a definite relationship between the 2 variables weight and concentration of sucrose. Any increase in sucrose concentration led to a decrease in weight. The prediction is supported by the manifest of the graph.Although there is one anomaly, all the other results stand out and give a uncoiled line of best fit exactly as I predicted. The point of early plasmolysis is where the concentration of sucrose and potato are even. There is no osmosis taking place at that point. As I said in my plan, if the water concentration is lower in the potato than in the sucrose solution, then water will pass from the sucrose solution into the potato, and it will gain weight. If there is a higher concentration of water in the potato, then the water will go out of the potato and into the sucrose solution.This is because osmosis is the come toment of water molecules from a region of high concentration to a region of low concentration, through a semi permeable membrane. The up raise of sucrose is the downfall of potato mass. IMAGE Osmosis In osmosis, water diffuses through a semi-permeable membrane. This diagram illustrates the concentrated sugar solution, isolated from dilute sucrose solution by a selectively permeable membrane. This has pores (holes) in it which are very fiddling, and selects what it wants to let through i. e. small molecules.Water molecules are very small. Each one is made of two total heat atoms and one oxygen atom. However, sugar molecules are more times larger. In potatoes, the pores of the membrane only let the water through. There is a higher concentration of sugar molecules on the respectable-hand side of the membrane in the diagram, than in the left wing-hand side. Sugar molecules would diffuse from the concentrated solution into the dilute one until they were as spread out if there was no membrane, but they cannot do this as the pores are too small for the sugar to get through the membrane.Therefore, the small water molecules diffuse into the concentrated sugar solution, to make it more dilute. This process is called osmosis. It is the diffusion of water molecules from a place where they are in a higher concentration, to a place where they are in a lower concentration, through a selectively permeable membrane. Potato cells plasmolyse in concentrated solutions. This diagram illustrates a dress cellIMAGE (which is similar to a potato cell) in a concentrated solution. It will lose water by osmosis. The cytoplasm and the vacuole will shrink.The cell membrane is semi-permeable and the vacuole contains a sucrose solution. So when a cell is laid in distilled water (high concentration) water will move across the semi-permeable membrane into the cell (lower water concentration) by osmosis, making the cell swell. This cell is called turgid. In potato cells, the cells would increase in length, stack and weight because of the extra water in the potato. If the potato was to be placed in a lower concentration, then the opposite would happen, because water would move out of the cell into the solution.If the solution is very concentrated, then a lot of water will diffuse out of the cell. The cytoplasm and vacuole will keep shrinking, but the cell protect will not as it is too stiff. As the cytoplasm shrinks further and further into the centre of the cell, the cell wall gets left behind. The cell membrane, surrounding the cytoplasm, separate away from the cell wall. If this happens, the cell is said to be plasmolysed. The potato will therefore, decrease in length, volume and weight. Plasmolysis is the point where the membrane is totally detached from its ell wall, and the potato is killed. Evaluation my experiment shows both(prenominal) accurate results. It c oncludes the experiment, and proves my prediction. My final examination results were quite reliable due to the precautions I took to make thisa fair test. The graph has a sequent slope pointing downwards, which is the clearest way to understand my prediction. All of them are not that closely together, neither far away, so a line of best fit joins some tips of the points, and causes it to go straight down.I have one nonsensical result, which falls on 0 on the x axis. This occurred in tube 5, which contained a concentration of 0. 4. perchance I did not carry out that properly. These were the of import problems in carrying out the experiment- * Scales kept pitiful * Difficult to get out the potato from the core * Potatoes get stuck in tube. * Solution is still left after wiping the potato and the scales * Different scales * couldnt control the temperature the point of early plasmolysis would not be valid.This is because I have just plotted it on the graph on a line which suits my results. To prove that it is the right point, I would have to do another experiment to find that out. The other evidence is likely to be valid, because as all the evidence links to the results. My investigation is fair because I did not change many things, expect the concentration of sucrose. I think that I have collected quite a lot of evidence to support my conclusion. The result table is the master(prenominal) source of evidence.I could try investigating with more percentages of sucrose concentration, the size of the potato and have more short intervals between the sucroses. This would have a more spread to the results, and therefore, results can be compared and evaluated more thoroughly. They would be very accurate as well as reliable. As I have an anomaly, my evidence may not be extremely correct. I would have to do more enquiry to what I have already done (in the conclusion) on osmosis, and see what very happens, and see if it occurs in plant cells all the time.If I were to repeat the experiment, I would use a very accurate scale, so that results would come out accurately and then I would have a accurate average. The graph will be fairly accurate. I would also make sure that the scale and the potato slices are properly wiped. This is because if they are not wiped, the weight would increase, and will not be accurate. I could use a syringe or a burette to measure the 10ml of sucrose solution, because those two apparatus are very accurate.I would do several more experiments with big sizes of potatoes to obtain more evidence to support my conclusion. I will also do some research on osmosis, and this will make me certain of my evidence if I have many to compare with. I will agree with the majority. Overall, I am very pleased with these results and with the evidence I have so far, that osmosis occurs when there is a high concentration and a low concentration, both aside a semi-permeable membrane. The lower concentrated substance diffuses through the membra ne to where there is the higher concentrated substance.